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pET28B-PU.1-ETS-Loop
(Plasmid #124551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)
  • Growth instructions
    Use BL21(DE3) for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PU.1 ETS domain (residues 167-272)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    330
  • Mutation
    Residues 216 to 223 replaced with corresponding sequence from murine Ets-1
  • GenBank ID
  • Entrez Gene
    Ets1 (a.k.a. D230050P06, Ets-1, Tpl1, p54, vs)
  • Entrez Gene
    Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
  • Tag / Fusion Protein
    • 6xHis tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28B-PU.1-ETS-Loop was a gift from Gregory Poon (Addgene plasmid # 124551 ; http://n2t.net/addgene:124551 ; RRID:Addgene_124551)

  • For your References section:

    Mapping interfacial hydration in ETS-family transcription factor complexes with DNA: a chimeric approach. Albrecht AV, Kim HM, Poon GMK. Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 10.1093/nar/gky894. 10.1093/nar/gky894 PubMed 30295801
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