-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEBG
-
Backbone manufacturerBruce Mayer
- Backbone size w/o insert (bp) 6100
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameUBP43
-
SpeciesH. sapiens (human)
-
MutationpEBG vector is from Bruce Mayer, may be different from other pEBG vectors in public domain.
-
Entrez GeneUSP18 (a.k.a. ISG43, PTORCH2, UBP43)
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEBG-hUBP43 was a gift from Dong-Er Zhang (Addgene plasmid # 12455 ; http://n2t.net/addgene:12455 ; RRID:Addgene_12455) -
For your References section:
UBP43 is a novel regulator of interferon signaling independent of its ISG15 isopeptidase activity. Malakhova OA, Kim KI, Luo JK, Zou W, Kumar KG, Fuchs SY, Shuai K, Zhang DE. EMBO J. 2006 Jun 7. 25(11):2358-67. 10.1038/sj.emboj.7601149 PubMed 16710296