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8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
(Plasmid #124540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    P1-EYFP-pA (Construct 5) (Addgene: 55197)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
  • gRNA/shRNA sequence
    Unknown
  • Species
    H. sapiens (human)
  • Mutation
    d11-41, d11-41
  • Promoter Minimal ADE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Engineered tRNA scaffold-flanked sgRNA containing 2xMS2 loops with a BsmBI cloning site for spacer cloning. The flanking variants show low/absent background with moderate guide release capability. Enhancer elements can be exchanged using the NheI site, and promoter/enhancer region can be changed using NcoI NheI double digestion.

Please visit https://www.biorxiv.org/content/10.1101/342485v1 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA was a gift from Tudor Fulga (Addgene plasmid # 124540 ; http://n2t.net/addgene:124540 ; RRID:Addgene_124540)

  • For your References section:

    Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression. Knapp DJHF, Michaels YS, Jamilly M, Ferry QRV, Barbosa H, Milne TA, Fulga TA. Nat Commun. 2019 Apr 2;10(1):1490. doi: 10.1038/s41467-019-09148-3. 10.1038/s41467-019-09148-3 PubMed 30940799
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