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Addgene

7xSMAD_RE::FLuc
(Plasmid #124531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AlphaB Vector
  • Backbone manufacturer
    Venken Lab, #124524
  • Backbone size w/o insert (bp) 2517
  • Total vector size (bp) 4780
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMAD FLuc reporter
  • Alt name
    Transcription Blocker + 7 copies of the SMAD DNA binding motif + miniP, Firefly luciferase
  • Species
    H. sapiens (human), Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer aat gag ctg att taa caa aaa ttt aac gcg
  • 3′ sequencing primer ctt ttt acg gtt cct ggc ctt ttg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert can be released with BsmBI for further cloning into Omega level vectors.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7xSMAD_RE::FLuc was a gift from Koen Venken (Addgene plasmid # 124531 ; http://n2t.net/addgene:124531 ; RRID:Addgene_124531)

  • For your References section:

    Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways. Sarrion-Perdigones A, Gonzalez Y, Venken KJT. Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. 10.1002/cpmb.121 PubMed 32539183
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