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TB:3xDBE_RE:MiniP
(Plasmid #124529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD
  • Backbone manufacturer
    Diego Orzaez Lab
  • Backbone size w/o insert (bp) 3057
  • Total vector size (bp) 3426
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3 copies of the Daf-16 family binding element
  • Alt name
    Transcription Blocker + 3 copies of the Daf-16 family binding element + miniP
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    369

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer gtcatagctgtttcctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert can be released with BsaI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TB:3xDBE_RE:MiniP was a gift from Koen Venken (Addgene plasmid # 124529 ; http://n2t.net/addgene:124529 ; RRID:Addgene_124529)

  • For your References section:

    Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways. Sarrion-Perdigones A, Gonzalez Y, Venken KJT. Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. 10.1002/cpmb.121 PubMed 32539183
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