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Addgene

Omega Destination-CMV::ELuc:bGH
(Plasmid #124528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1 derived
  • Backbone manufacturer
    Venken Lab, self made
  • Backbone size (bp) 5000
  • Modifications to backbone
    Includes CMV:ELuc:bGH expression unit
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer aat gag ctg att taa caa aaa ttt aac gcg
  • 3′ sequencing primer ctt ttt acg gtt cct ggc ctt ttg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Destination vector. BsmBI cloning site. Pink/White selection

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Omega Destination-CMV::ELuc:bGH was a gift from Koen Venken (Addgene plasmid # 124528 ; http://n2t.net/addgene:124528 ; RRID:Addgene_124528)

  • For your References section:

    Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways. Sarrion-Perdigones A, Gonzalez Y, Venken KJT. Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. 10.1002/cpmb.121 PubMed 32539183
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