AlphaE
(Plasmid
#124527)
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Purpose(Empty Backbone) pColE1_AlphaE
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneColE1 derived
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Backbone manufacturerVenken Lab, self made
- Backbone size (bp) 2330
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer aat gag ctg att taa caa aaa ttt aac gcg
- 3′ sequencing primer ctt ttt acg gtt cct ggc ctt ttg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a new GB2.0-type vector, that expands the Alpha level. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AlphaE was a gift from Koen Venken (Addgene plasmid # 124527 ; http://n2t.net/addgene:124527 ; RRID:Addgene_124527) -
For your References section:
Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways. Sarrion-Perdigones A, Gonzalez Y, Venken KJT. Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. 10.1002/cpmb.121 PubMed 32539183
