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nifF in pET21b
(Plasmid #124520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5403
  • Total vector size (bp) 5950
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 (DE3) for expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flavodoxin II
  • Alt name
    nifF
  • Species
    Azotobacter vinelandii
  • Insert Size (bp)
    543
  • GenBank ID
  • Entrez Gene
    nifF (a.k.a. AvCA_01710)
  • Promoter T7 / lac
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nifF in pET21b was a gift from Douglas Rees (Addgene plasmid # 124520 ; http://n2t.net/addgene:124520 ; RRID:Addgene_124520)

  • For your References section:

    Electrochemical and structural characterization of Azotobacter vinelandii flavodoxin II. Segal HM, Spatzal T, Hill MG, Udit AK, Rees DC. Protein Sci. 2017 Oct;26(10):1984-1993. doi: 10.1002/pro.3236. Epub 2017 Aug 30. 10.1002/pro.3236 PubMed 28710816
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