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pTRE-tight2-Tmem98-FLAG
(Plasmid #124506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTretight-2
  • Backbone manufacturer
    https://www.addgene.org/19407/
  • Backbone size w/o insert (bp) 4200
  • Total vector size (bp) 4900
  • Modifications to backbone
    an SV40-puromycin-polyA resistance cassette was inserted into the backbone to allow selection in mammalian cells
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tmem98
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    770
  • Entrez Gene
    Tmem98 (a.k.a. 6530411B15Rik, AI463522)
  • Promoter tetO-CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40 polyA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-tight2-Tmem98-FLAG was a gift from Renee van Amerongen (Addgene plasmid # 124506 ; http://n2t.net/addgene:124506 ; RRID:Addgene_124506)

  • For your References section:

    TMEM98 is a negative regulator of FRAT mediated Wnt/ss-catenin signalling. van der Wal T, Lambooij JP, van Amerongen R. PLoS One. 2020 Jan 21;15(1):e0227435. doi: 10.1371/journal.pone.0227435. eCollection 2020. 10.1371/journal.pone.0227435 PubMed 31961879
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