pCDNA3.1-Tmem98deltaN-FLAG
(Plasmid #124502)

• Purpose: Expresses an N-terminally truncated version of FLAG-tagged mouse TMEM98 in mammalian cells (C-terminal tag)
• Depositing Lab: Renee van Amerongen
• Publication: van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. doi: 10.1371/journal.pone.0227435. eCollection 2020.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tmem98
• Species: M. musculus (mouse)
• Insert Size (bp): 670
• Entrez Gene: Tmem98 (a.k.a. 6530411B15Rik, AI463522)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Bgh polyA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint