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Addgene

pSuperRetro-EFP siRNA2
(Plasmid #12450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuperRetroPuro
  • Backbone size w/o insert (bp) 6349
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    siRNA against EFP
  • Alt name
    EFP
  • Species
    H. sapiens (human)
  • Entrez Gene
    TRIM25 (a.k.a. EFP, RNF147, Z147, ZNF147)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pBluescript KS
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The targeted sequence for siRNA is EFP cDNA region 711-729 (ggtggagcagctacaacaa). The synthesized oligonucleotides is 5'-Gatccccggtggagcagctacaacaattcaag agattgttgtagctgctccaccttttta-3' and 5'-Agcttaaaaaggtggagcagctacaacaatct cttgaattgttgtagctgctccaccggg-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuperRetro-EFP siRNA2 was a gift from Dong-Er Zhang (Addgene plasmid # 12450 ; http://n2t.net/addgene:12450 ; RRID:Addgene_12450)

  • For your References section:

    The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. Zou W, Zhang DE. J Biol Chem. 2006 Feb 17. 281(7):3989-94. 10.1074/jbc.M510787200 PubMed 16352599
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