6559 pJC25YDJ1 #7
(Plasmid
#1245)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJC25
- Backbone size w/o insert (bp) 3654
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYdj1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneYDJ1 (a.k.a. YNL064C, HSP40, MAB3, MAS5)
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Tag
/ Fusion Protein
- his tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site nde1 (not destroyed)
- 3′ cloning site nde1 (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJohn Glover
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6559 pJC25YDJ1 #7 was a gift from Susan Lindquist (Addgene plasmid # 1245 ; http://n2t.net/addgene:1245 ; RRID:Addgene_1245) -
For your References section:
Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Glover JR, Lindquist S. Cell 1998 Jul 10;94(1):73-82. 10.1016/S0092-8674(00)81223-4 PubMed 9674429