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Addgene

hKCNB1 F416L
(Plasmid #124491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHA-CIneo (Promega’s pCI-neo vector modified with a HA-tag)
  • Backbone manufacturer
    promega
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human KCNB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2578
  • Mutation
    changed phenyalanine 416 to leucine
  • Entrez Gene
    KCNB1 (a.k.a. DRK1, Kv2.1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer agctgaattccaccatgccggcgggcatgacg
  • 3′ sequencing primer TGGGTAGAATTCGATGCTCTGATCTCGTGTGCTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hKCNB1 F416L was a gift from Federico Sesti (Addgene plasmid # 124491 ; http://n2t.net/addgene:124491 ; RRID:Addgene_124491)

  • For your References section:

    Complexes formed with integrin-alpha5 and KCNB1 potassium channel wild type or epilepsy-susceptibility variants modulate cellular plasticity via Ras and Akt signaling. Yu W, Shin MR, Sesti F. FASEB J. 2019 Dec;33(12):14680-14689. doi: 10.1096/fj.201901792R. Epub 2019 Nov 2. 10.1096/fj.201901792R PubMed 31682765
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