pFlagCMV2-EFP
(Plasmid #12449)

• Depositing Lab: Dong-Er Zhang
• Publication: Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94.

Backbone

• Vector backbone: pFlag-CMV2
• Backbone size w/o insert (bp): 4679
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EFP
• Species: H. sapiens (human)
• Entrez Gene: TRIM25 (a.k.a. EFP, RNF147, Z147, ZNF147)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human EFP cDNA subcloned into the blunt ended HindIII site of pFlag-CMV2.