FoxO6deltaCt
(Plasmid
#124486)
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PurposeExpresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepECE (Addgene Plasmid 26453)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFoxO6
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SpeciesM. musculus (mouse)
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Insert Size (bp)1180
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MutationT26A, S184A, transactivation domain deleted from residue 388
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Entrez GeneFoxo1 (a.k.a. Afxh, FKHR, Fkhr1, Foxo1a)
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Entrez GeneFoxo4 (a.k.a. Afxh, Mllt7, afx)
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Entrez GeneFoxo3 (a.k.a. 1110048B16Rik, 2010203A17Rik, FKHRL1, Fkhr2, Foxo3a)
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Entrez GeneFoxo6
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FoxO6deltaCt was a gift from Anne Brunet (Addgene plasmid # 124486 ; http://n2t.net/addgene:124486 ; RRID:Addgene_124486) -
For your References section:
FoxO6 regulates memory consolidation and synaptic function. Salih DA, Rashid AJ, Colas D, de la Torre-Ubieta L, Zhu RP, Morgan AA, Santo EE, Ucar D, Devarajan K, Cole CJ, Madison DV, Shamloo M, Butte AJ, Bonni A, Josselyn SA, Brunet A. Genes Dev. 2012 Dec 15;26(24):2780-801. doi: 10.1101/gad.208926.112. Epub 2012 Dec 7. 10.1101/gad.208926.112 PubMed 23222102