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pET15b-mISG15(GG)
(Plasmid #12448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C-terminal processed form of ISG15
  • Alt name
    ISG15
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-mISG15(GG) was a gift from Dong-Er Zhang (Addgene plasmid # 12448 ; http://n2t.net/addgene:12448 ; RRID:Addgene_12448)

  • For your References section:

    UBP43, an ISG15-specific deconjugating enzyme: expression, purification, and enzymatic assays. Kim KI, Zhang DE. Methods Enzymol. 2005 . 398():491-9. 10.1016/S0076-6879(05)98040-3 PubMed 16275353
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