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Addgene

pSPgRNA_BLK_E2-3
(Plasmid #124476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSPgRNA (Addgene #47108)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BLK_E2-3_gRNA
  • Alt name
    BLK_LA35
  • gRNA/shRNA sequence
    TCTGCATTAGAGGGCCTAGC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Entrez Gene
    BLK (a.k.a. MODY11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer hU6-F
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPgRNA_BLK_E2-3 was a gift from Iouri Chepelev (Addgene plasmid # 124476 ; http://n2t.net/addgene:124476 ; RRID:Addgene_124476)

  • For your References section:

    Haplotype-specific chromatin looping reveals genetic interactions of regulatory regions modulating gene expression in 8p23.1. Saint Just Ribeiro M, Tripathi P, Namjou B, Harley JB, Chepelev I. Front Genet. 2022 Sep 7;13:1008582. doi: 10.3389/fgene.2022.1008582. eCollection 2022. 10.3389/fgene.2022.1008582 PubMed 36160011
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