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PurposeExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlipCherry-T2A-GFP
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FlipCherry-T2A-GFP was a gift from Xiaokun Shu (Addgene plasmid # 124436 ; http://n2t.net/addgene:124436 ; RRID:Addgene_124436) -
For your References section:
Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in animals. Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X. J Am Chem Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042. 10.1021/jacs.8b13042 PubMed 30821975