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PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
(Plasmid #124431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Vector type
    zebrafish (if expression level is too high in zebrafish, it may have toxicity due to two T2As)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FlipGFP(Casp3 cleavage seq) T2A mCherry
  • Species
    Synthetic

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry was a gift from Xiaokun Shu (Addgene plasmid # 124431 ; http://n2t.net/addgene:124431 ; RRID:Addgene_124431)

  • For your References section:

    Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in animals. Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X. J Am Chem Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042. 10.1021/jacs.8b13042 PubMed 30821975
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