pCAGGS-FLAG-mISG15
(Plasmid #12443)

• Depositing Lab: Dong-Er Zhang
• Publication: Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-600.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4790
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ISG15
• Species: M. musculus (mouse)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: pCAG-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the ISG15 insert ends with the following amino acid sequence, LRLRGG. It is from pre-ISG15 which has a C-terminal deletion when compared to ISG15 sequences in GenBank. This plasmid is exactly as described in the associated publication listed below.