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Addgene

aCKAR
(Plasmid #124427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aCKAR
  • Species
    Synthetic
  • Insert Size (bp)
    1998
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCFP (N terminal on insert)
    • mCitrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aCKAR was a gift from Alexandra Newton (Addgene plasmid # 124427 ; http://n2t.net/addgene:124427 ; RRID:Addgene_124427)

  • For your References section:

    Activation of atypical protein kinase C by sphingosine 1-phosphate revealed by an aPKC-specific activity reporter. Kajimoto T, Caliman AD, Tobias IS, Okada T, Pilo CA, Van AN, Andrew McCammon J, Nakamura SI, Newton AC. Sci Signal. 2019 Jan 1;12(562). pii: 12/562/eaat6662. doi: 10.1126/scisignal.aat6662. 10.1126/scisignal.aat6662 PubMed 30600259
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