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Addgene

pFlagCMV2-UbcM8C86A
(Plasmid #12441)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12441 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlag-CMV2
  • Backbone size w/o insert (bp) 4679
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcM8
  • Species
    M. musculus (mouse)
  • Mutation
    C86A
  • Entrez Gene
    Ube2l6 (a.k.a. 2810489I21Rik, RIG-B, UBCH8, Ubce8, Ubcm8)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCEP fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlagCMV2-UbcM8C86A was a gift from Dong-Er Zhang (Addgene plasmid # 12441 ; http://n2t.net/addgene:12441 ; RRID:Addgene_12441)

  • For your References section:

    Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE. Mol Cell Biol. 2004 Nov . 24(21):9592-600. 10.1128/MCB.24.21.9592-9600.2004 PubMed 15485925
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