Gal4-TET1-878-911-L897A(pL84)
(Plasmid
#124401)
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PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLeicester 84
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Backbone manufacturerUniversity of Leicester
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGal4-TET1-878-911(L897A mutation)
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SpeciesH. sapiens (human)
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Insert Size (bp)228
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MutationL897A
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Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
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Tag
/ Fusion Protein
- gal4 dna binding domain (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gal4-TET1-878-911-L897A(pL84) was a gift from Shaun Cowley (Addgene plasmid # 124401 ; http://n2t.net/addgene:124401 ; RRID:Addgene_124401) -
For your References section:
Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain. Chandru A, Bate N, Vuister GW, Cowley SM. Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w. 10.1038/s41598-018-32942-w PubMed 30279502