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Addgene

FLAG-FullLength-TET1-CXXC/LLAA
(Plasmid #124399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLeicester 38
  • Backbone manufacturer
    University of Leicester
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6500
  • Mutation
    CXXC zinc finger deletion (aa 585-624) and L897A/L900A mutations (full length protein is 2136 aa)
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-FullLength-TET1-CXXC/LLAA was a gift from Shaun Cowley (Addgene plasmid # 124399 ; http://n2t.net/addgene:124399 ; RRID:Addgene_124399)

  • For your References section:

    Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain. Chandru A, Bate N, Vuister GW, Cowley SM. Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w. 10.1038/s41598-018-32942-w PubMed 30279502
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