FLAG-FullLength-TET1-WT(21)
(Plasmid
#124396)
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PurposeFull-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLeicester 38
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Backbone manufacturerUniversity of Leicester
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLAG-FullLength-TET1
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SpeciesH. sapiens (human)
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Insert Size (bp)6500
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Mutationfull length protein is 2136 aa
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Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-FullLength-TET1-WT(21) was a gift from Shaun Cowley (Addgene plasmid # 124396 ; http://n2t.net/addgene:124396 ; RRID:Addgene_124396) -
For your References section:
Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain. Chandru A, Bate N, Vuister GW, Cowley SM. Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w. 10.1038/s41598-018-32942-w PubMed 30279502
Map uploaded by the depositor.
