pCDNA3-HA PSPC1 Y523F
(Plasmid
#124373)
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PurposeExpresses PSPC1 Y523F in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 7018
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSPC1
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SpeciesH. sapiens (human)
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MutationY523F
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Entrez GenePSPC1 (a.k.a. PSP1)
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Tag
/ Fusion Protein
- HA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For wildtype PSPC1 see https://www.addgene.org/101764/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-HA PSPC1 Y523F was a gift from Yuh-Shan Jou (Addgene plasmid # 124373 ; http://n2t.net/addgene:124373 ; RRID:Addgene_124373) -
For your References section:
PSPC1-interchanged interactions with PTK6 and beta-catenin synergize oncogenic subcellular translocations and tumor progression. Lang YD, Chen HY, Ho CM, Shih JH, Hsu EC, Shen R, Lee YC, Chen JW, Wu CY, Yeh HW, Chen RH, Jou YS. Nat Commun. 2019 Dec 16;10(1):5716. doi: 10.1038/s41467-019-13665-6. 10.1038/s41467-019-13665-6 PubMed 31844057