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Addgene

pAV-U6+27-Tornado-F30-Broccoli-empty
(Plasmid #124361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124361 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please note that this plasmid is prone to recombination. We recommend screening 3-5 single colonies upon receiving your sample.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tornado-F30-Broccoli-empty
  • Promoter U6+27

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer F_U6
  • 3′ sequencing primer R_MCS
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV-U6+27-Tornado-F30-Broccoli-empty was a gift from Samie Jaffrey (Addgene plasmid # 124361)

  • For your References section:

    Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Litke JL, Jaffrey SR. Nat Biotechnol. 2019 Apr 8. pii: 10.1038/s41587-019-0090-6. doi: 10.1038/s41587-019-0090-6. 10.1038/s41587-019-0090-6 PubMed 30962542
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