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Addgene

pBHDuet40
(Plasmid #124358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3992
  • Total vector size (bp) 4256
  • Modifications to backbone
    His6-GB1 tag, deletion of MCS-2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gp41-1 N-intein
  • Species
    metagenomic
  • Insert Size (bp)
    264
  • GenBank ID
    AACY020177900
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GB1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHDuet40 was a gift from Hideo Iwai (Addgene plasmid # 124358 ; http://n2t.net/addgene:124358 ; RRID:Addgene_124358)

  • For your References section:

    Off-pathway-sensitive protein splicing screening based on a toxin/antitoxin system. Beyer HM, Iwai H. Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139. 10.1002/cbic.201900139 PubMed 30963690
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