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MigR1-AE9aP
(Plasmid #12434)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12434 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MigR1
  • Backbone manufacturer
    See comments
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AML1-ETO9a as in t(8;21) patients
  • Alt name
    AML1
  • Alt name
    ETO
  • Species
    H. sapiens (human)
  • Mutation
    AML1-ETO9a cDNA as in t(8;21) patients
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
  • Entrez Gene
    RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See paper (not destroyed)
  • 3′ cloning site See paper (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MigR1-AE9aP was a gift from Dong-Er Zhang (Addgene plasmid # 12434 ; http://n2t.net/addgene:12434 ; RRID:Addgene_12434)

  • For your References section:

    A previously unidentified alternatively spliced isoform of t(8;21) transcript promotes leukemogenesis. Yan M, Kanbe E, Peterson LF, Boyapati A, Miao Y, Wang Y, Chen IM, Chen Z, Rowley JD, Willman CL, Zhang DE. Nat Med. 2006 Aug . 12(8):945-9. 10.1038/nm1443 PubMed 16892037
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