MigR1-AEtr
(Plasmid #12432)

• Depositing Lab: Dong-Er Zhang
• Publication: Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 101(49):17186-91.

Backbone

• Vector backbone: MigR1
• Backbone manufacturer: See comments
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C-term truncated AML1-ETO
• Alt name: AML1
• Alt name: ETO
• Species: H. sapiens (human)
• Entrez Gene: RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
• Entrez Gene: RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See comments (not destroyed)
• 3′ cloning site: See comments (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: (HA)-tagged AML1-ETO was a gift from M. Ohki, National Cancer Center Research Institute, Tokyo.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".

The C-terminal truncated AML1-ETO expression retroviral construct was created by inserting the BamHI and blunted XbaI fragment of 3' end of mutated HA-AML1-ETO cDNA from the AML mouse into BamHI and blunt-ended EcoRI sites in Mig-A/E to form Mig-A/Etr.