pLC-HA active Nedd8-P2A-Hygro
(Plasmid #124307)

• Purpose: Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter
• Depositing Lab: Eva Gottwein
• Publication: Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732.

Backbone

• Vector backbone: pLCE
• Total vector size (bp): 7803
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nedd8
• Species: H. sapiens (human)
• Insert Size (bp): 225
• Mutation: Five C-terminal amino acids missing compared to wild-type Nedd8. The cleaved form represents the active form of Nedd8.
• Entrez Gene: NEDD8 (a.k.a. NEDD-8)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLC HA-acNedd8_P2A_Hygro.gb
• A portion of this plasmid was derived from a plasmid made by: Nedd8 cDNA from Addgene plasmid 18711 was used as a template for PCR amplification and subsequent mutagenesis.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry