pLC-Flag-CRBN-P2A-Hygro
(Plasmid #124303)

• Purpose: Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter
• Depositing Lab: Eva Gottwein
• Publication: Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732.

Backbone

• Vector backbone: pLCE
• Total vector size (bp): 8895
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRBN
• Alt name: Cereblon
• Species: H. sapiens (human)
• Insert Size (bp): 1347
• Entrez Gene: CRBN (a.k.a. MRT2, MRT2A)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLC Flag-CRBN_P2A_Hygro.gb
• A portion of this plasmid was derived from a plasmid made by: CRBN cDNA was PCR amplified from Addgene plasmid 107380.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry