pLC-Flag-UBE2G1 sg2R-P2A-Hygro
(Plasmid #124299)

• Purpose: Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.
• Depositing Lab: Eva Gottwein
• Publication: Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732.

Backbone

• Vector backbone: pLCE
• Total vector size (bp): 8082
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBE2G1
• Species: H. sapiens (human)
• Insert Size (bp): 534
• Mutation: several silent mutations to disrupt sgRNA targeting
• GenBank ID: NM_003342
• Entrez Gene: UBE2G1 (a.k.a. E217K, UBC7, UBE2G)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLC Flag-UBE2G1_P2A_Hygro- sgRNA D2 resistant.gb
• A portion of this plasmid was derived from a plasmid made by: UBE2G1 cDNA was cloned from Addgene plasmid 31407

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry