pSLC-284
(Plasmid
#124294)
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PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepANT4
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Backbone manufacturerStanley Falkow (PMID 9673287)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namevsfGFP-9
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A 9aa linker in vGFP-9 enables reflexive binding of the Enhancer domain to the fused GFP domain.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLC-284 was a gift from Swaine Chen (Addgene plasmid # 124294 ; http://n2t.net/addgene:124294 ; RRID:Addgene_124294) -
For your References section:
Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP. Eshaghi M, Mehershahi KS, Chen SL. Pathogens. 2016 Jan 5;5(1). pii: pathogens5010003. doi: 10.3390/pathogens5010003. 10.3390/pathogens5010003 PubMed 26742079
