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pPICZ-mouseGIRK2-eGFP-StII-6xHis
(Plasmid #124277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZ
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GIRK2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Kcnj6 (a.k.a. BIR1, GIRK2, KATP2, KCNJ7, Kir3.2, weaver, wv)
  • Promoter AOX1
  • Tag / Fusion Protein
    • eGFP-StreptagII-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAC TGG TTC CAA TTG ACA AGC
  • 3′ sequencing primer GCA AAT GGC ATT CTG ACA TCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPICZ-mouseGIRK2-eGFP-StII-6xHis was a gift from Arthur Laganowsky (Addgene plasmid # 124277 ; http://n2t.net/addgene:124277 ; RRID:Addgene_124277)

  • For your References section:

    Selective binding of a toxin and phosphatidylinositides to a mammalian potassium channel. Liu Y, LoCaste CE, Liu W, Poltash ML, Russell DH, Laganowsky A. Nat Commun. 2019 Mar 22;10(1):1352. doi: 10.1038/s41467-019-09333-4. 10.1038/s41467-019-09333-4 PubMed 30902995
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