pMSP1D12W/2Y
(Plasmid #124275)

• Purpose: Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
• Depositing Lab: Alexey S. Ladokhin
• Publication: Bacterial expression plasmid for production of recombinant MSP1D12W/2Y protein where 2 tryptophan residues are substituted with 2 tyrosines (unpublished)

Backbone

• Vector backbone: pET28b
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 6600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Membrane scaffold protein
• Species: Synthetic
• Insert Size (bp): 600
• Mutation: Two tryptophans changed to two tyrosines
• Promoter: T7
• Tag / Fusion Protein:
  • 7xHis-tag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We got the original pMSP1D1 from Addgene and introduced two mutations 2W/2Y.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry