Lamin A (R435C)-mRFP
(Plasmid
#124274)
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PurposeLamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemRFP-N2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLMNA (R435C)
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SpeciesH. sapiens (human)
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MutationLMNA (R435C)
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Entrez GeneLMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
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Tag
/ Fusion Protein
- mRFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byLamin A mutants were cloned by Peter Meinke 2018 (Ludwig-Maximilians-University of Munich). mRFP was a gift from Anne Straub in 2005
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lamin A (R435C)-mRFP was a gift from Eric Schirmer (Addgene plasmid # 124274 ; http://n2t.net/addgene:124274 ; RRID:Addgene_124274)