Lamin A (R377H)-mRFP
(Plasmid #124270)

• Purpose: Lamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
• Depositing Lab: Eric Schirmer
• Publication: Schirmer lab plasmids (unpublished)

Backbone

• Vector backbone: mRFP-N2
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LMNA (R377H)
• Species: H. sapiens (human)
• Mutation: LMNA (R377H)
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Tag / Fusion Protein:
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lamin A mutants were cloned by Peter Meinke 2018 (Ludwig-Maximilians-University of Munich). mRFP was a gift from Anne Straub in 2005

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry