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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12427 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4657
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBF beta
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SpeciesM. musculus (mouse)
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Entrez GeneCbfb (a.k.a. AI893578, PEA2, PEBP2b, Pebp2, Pebpb2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer pCEP fwd (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5-CBFbeta was a gift from Scott Hiebert (Addgene plasmid # 12427 ; http://n2t.net/addgene:12427 ; RRID:Addgene_12427) -
For your References section:
The t(8;21) fusion protein interferes with AML-1B-dependent transcriptional activation. Meyers S, Lenny N, Hiebert SW. Mol Cell Biol. 1995 Apr . 15(4):1974-82. PubMed 7891692