NLS-mRFP-V1
(Plasmid
#124266)
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PurposeNuclear localised mRFP reporter construct with one half of split Venus
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124266 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- Venus1 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bymRFP was a gift from Anne Straub in 2005. Split Venus plasmids (V1/V2) were a gift from Patrick Heun 2018 (WCCB, Edinburgh)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NLS-mRFP-V1 was a gift from Eric Schirmer (Addgene plasmid # 124266 ; http://n2t.net/addgene:124266 ; RRID:Addgene_124266)
