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PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTMEM173
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Alt nameSTING
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Alt nameNET23
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SpeciesH. sapiens (human)
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Entrez GeneSTING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
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Tag
/ Fusion Protein
- Venus1 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySTING IMAGE clone came from RZPD - German Science Centre for Genome Research in 2004. Split Venus plasmids (V1/V2) were a gift from Patrick Heun 2018 (WCCB, Edinburgh)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STING-V1 was a gift from Eric Schirmer (Addgene plasmid # 124262 ; http://n2t.net/addgene:124262 ; RRID:Addgene_124262)