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pKT-IDH1(R132H)-IRES-Katushka
(Plasmid #124257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKT
  • Backbone size w/o insert (bp) 4891
  • Total vector size (bp) 6149
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Isocitrate dehydrogenase 1 (R132H)
  • Alt name
    IDH1 (R132H)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1258
  • Mutation
    Arginine 132 is mutated to Histidine. This mutation is found in Low Grade Gliomas and produces the oncometabolite, 2-Hydroxyglutarate.
  • Entrez Gene
    IDH1 (a.k.a. HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT-IDH1(R132H)-IRES-Katushka was a gift from Maria Castro (Addgene plasmid # 124257 ; http://n2t.net/addgene:124257 ; RRID:Addgene_124257)

  • For your References section:

    IDH1-R132H acts as a tumor suppressor in glioma via epigenetic up-regulation of the DNA damage response. Nunez FJ, Mendez FM, Kadiyala P, Alghamri MS, Savelieff MG, Garcia-Fabiani MB, Haase S, Koschmann C, Calinescu AA, Kamran N, Saxena M, Patel R, Carney S, Guo MZ, Edwards M, Ljungman M, Qin T, Sartor MA, Tagett R, Venneti S, Brosnan-Cashman J, Meeker A, Gorbunova V, Zhao L, Kremer DM, Zhang L, Lyssiotis CA, Jones L, Herting CJ, Ross JL, Hambardzumyan D, Hervey-Jumper S, Figueroa ME, Lowenstein PR, Castro MG. Sci Transl Med. 2019 Feb 13;11(479). pii: 11/479/eaaq1427. doi: 10.1126/scitranslmed.aaq1427. 10.1126/scitranslmed.aaq1427 PubMed 30760578
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