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Addgene

pMCSF-R(m40)-luc
(Plasmid #12425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXP2
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M-CSF receptor promoter fragment
  • Alt name
    M-CSF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Mutation
    PU.1 binding site mutation (created a KpnI site in mutation site)
  • Entrez Gene
    CSF1R (a.k.a. BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, GPSC, HDLS, HDLS1, M-CSF-R)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSF-R(m40)-luc was a gift from Dong-Er Zhang (Addgene plasmid # 12425 ; http://n2t.net/addgene:12425 ; RRID:Addgene_12425)

  • For your References section:

    The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor. Zhang DE, Hetherington CJ, Chen HM, Tenen DG. Mol Cell Biol. 1994 Jan . 14(1):373-81. PubMed 8264604
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