pDOE11 Lat52:mCherry transient
(Plasmid
#124249)
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Purpose(Empty Backbone) Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDOE11
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description of the original pDOE vector system PMID: 25187041.
Plasmid citation:
pDOE11 Lat52:mCherry transient (modifed from pDOE11; Gookin and Assmann 2014 Plant J. 80:553-67) was a gift from Attila Fehér (Addgene plasmid # 124248).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDOE11 Lat52:mCherry transient was a gift from Attila Fehér (Addgene plasmid # 124249 ; http://n2t.net/addgene:124249 ; RRID:Addgene_124249) -
For your References section:
In silico identification and experimental validation of amino acid motifs required for the Rho-of-plants GTPase-mediated activation of receptor-like cytoplasmic kinases. Lajko DB, Valkai I, Domoki M, Menesi D, Ferenc G, Ayaydin F, Feher A. Plant Cell Rep. 2018 Apr;37(4):627-639. doi: 10.1007/s00299-018-2256-y. Epub 2018 Jan 16. 10.1007/s00299-018-2256-y PubMed 29340786
