pDOE20 mC VN
(Plasmid
#124246)
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Purpose(Empty Backbone) Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in Agrobacterium- transformed plant cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDOE20
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note this plasmid contains a IS5 transposable element. Plasmid works for depositor but it is possible element could cause an issue.
Description of the original pDOE vector system PMID: 25187041.
Plasmid citation:
pDOE20 mC VN (modifed from pDOE20; Gookin and Assmann 2014 Plant J. 80:553-67) was a gift from Attila Fehér (Addgene plasmid # 124246).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDOE20 mC VN was a gift from Attila Fehér (Addgene plasmid # 124246 ; http://n2t.net/addgene:124246 ; RRID:Addgene_124246)
