Moe-pDONR221
(Plasmid
#124239)
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PurposeGateway entry clone of Drosophila Moesin cDNA encoding the D isoform.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
- Backbone size w/o insert (bp) 2555
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrosophila Moesin
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Alt namedMoe
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Alt nameCG10701
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1728
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Entrez GeneMoe (a.k.a. Dmel_CG10701, CG10701, D17, DMoe, Dmel\CG10701, Dmoe, EMR1, ERM, Emr1, GMA, Moes, anon-WO03040301.155, anon-WO03040301.157, anon-WO03040301.159, anon-WO03040301.161, dMoe, gma, l(1)G0067, l(1)G0323, l(1)G0404, l(1)G0415, moe, moesin, moesin/ezrin/radixin homolog mRNA)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDGC Gold cDNA collection, BDGP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Moe-pDONR221 was a gift from Peter Vilmos (Addgene plasmid # 124239 ; http://n2t.net/addgene:124239 ; RRID:Addgene_124239) -
For your References section:
The actin-binding ERM protein Moesin directly regulates spindle assembly and function during mitosis. Vilmos P, Kristo I, Szikora S, Jankovics F, Lukacsovich T, Kari B, Erdelyi M. Cell Biol Int. 2016 Jun;40(6):696-707. doi: 10.1002/cbin.10607. Epub 2016 Apr 6. 10.1002/cbin.10607 PubMed 27006187