pPWCh
(Plasmid
#124238)
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Purpose(Empty Backbone) Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124238 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPW
- Backbone size (bp) 12314
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Vector typeInsect Expression
- Promoter UASp
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPWCh was a gift from Peter Vilmos (Addgene plasmid # 124238 ; http://n2t.net/addgene:124238 ; RRID:Addgene_124238) -
For your References section:
The actin-binding ERM protein Moesin directly regulates spindle assembly and function during mitosis. Vilmos P, Kristo I, Szikora S, Jankovics F, Lukacsovich T, Kari B, Erdelyi M. Cell Biol Int. 2016 Jun;40(6):696-707. doi: 10.1002/cbin.10607. Epub 2016 Apr 6. 10.1002/cbin.10607 PubMed 27006187
