CLYBL-TO-hNIL-BSD-mApple
(Plasmid #124230)

• Purpose: Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
• Depositing Lab: Michael Ward
• Publication: Next generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons (unpublished)

Backbone

• Vector backbone: CLYBL targeting
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NGN2, ISL1, LHX3
• Species: H. sapiens (human)
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
• Entrez Gene: ISL1 (a.k.a. ISLET1, Isl-1)
• Entrez Gene: LHX3 (a.k.a. CPHD3, LIM3, M2-LHX3)

Resource Information

• Supplemental Documents:
  • J37 CLYBL-TO-hNIL-BSD-mApple.gbk
  • Protocol for lipid-based RNP transfection of iPSCs for integration of safe-harbor targeting vectors

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry