pMCSF-R(dAB)-luc
(Plasmid
#12423)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXP2
- Backbone size w/o insert (bp) 5900
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM-CSF receptor promoter fragment
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Alt nameM-CSF
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SpeciesH. sapiens (human)
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Insert Size (bp)400
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MutationNo AML1 and C/EBP binding sites (delete bp -86 to 62)(created a KpnI site in the mutation site)
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Entrez GeneCSF1 (a.k.a. CSF-1, MCSF, PG-M-CSF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSF-R(dAB)-luc was a gift from Dong-Er Zhang (Addgene plasmid # 12423 ; http://n2t.net/addgene:12423 ; RRID:Addgene_12423) -
For your References section:
CCAAT enhancer-binding protein (C/EBP) and AML1 (CBF alpha2) synergistically activate the macrophage colony-stimulating factor receptor promoter. Zhang DE, Hetherington CJ, Meyers S, Rhoades KL, Larson CJ, Chen HM, Hiebert SW, Tenen DG. Mol Cell Biol. 1996 Mar . 16(3):1231-40. PubMed 8622667