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Addgene

mNG-mCh
(Plasmid #124215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSAV057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Add 0.5% glucose to repress expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mNG-mCh
  • Alt name
    pNM002

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: mNeonGreen contains a M1-N9 amino acid deletion that allows for better expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNG-mCh was a gift from Tanneke den Blaauwen (Addgene plasmid # 124215 ; http://n2t.net/addgene:124215 ; RRID:Addgene_124215)

  • For your References section:

    Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli. Meiresonne NY, van der Ploeg R, Hink MA, den Blaauwen T. MBio. 2017 Sep 12;8(5). pii: mBio.01089-17. doi: 10.1128/mBio.01089-17. 10.1128/mBio.01089-17 PubMed 28900026
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